spoVAF [2019-06-19 12:58:29]

essential for the uptake of the 1:1 chelate of pyridine-2,6-dicarboxylic acid (DPA(2,6)) and Ca(2 ) into developing spores, required for spore maturation

locus: BSU23390

pI: 6.21

mw: 55.44 kDa

protein length: 493 aa

gene length: 1482 bp

function: spore maturation

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 2,438,372 → 2,439,853

Phenotypes of a mutant

• delayed germination with germinant receptor-dependent germinants [Pubmed|24682327]

The protein

Protein family

• gerABKA family (according to Swiss-Prot)

[SW|Localization]

• integral inner spore membrane protein [Pubmed|24682327]

Expression and Regulation

Operons

• genes: [gene|5410018998EC9792A69CF0D9C438EEF5AC8A82C5|spoVAA]-[gene|AEC70413BDC29F435776D532E7049AA9A0BD893D|spoVAB]-[gene|B280951A232E941FB0A6C8834049765BE1E16437|spoVAC]-[gene|9531F0A0DC4AE5696FFBAC9C2A773F15840E3255|spoVAD]-[gene|31D6CB7EC20C39C1815CA4552B8B9C4BE304E86B|spoVAEB]-[gene|B44529B69769D7AF1010E06C95E398EA91A3E798|spoVAEA]-[gene|CBE5093DFBBCE25071CCE12408BC9AFF7A158C03|spoVAF]-[gene|02F1C926D200DE85C93A0ACEF23AD25FBD12B0BD|lysA]
  description: [Pubmed|7934830]

  sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|15699190,1903432], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

  regulatory mechanism

  • [protein|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|SpoVT]: activation, [Pubmed|8755877], in [regulon|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|SpoVT regulon]

  regulation

  • expressed late during sporulation in the forespore ([protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG], [SW|SpoVT]) [Pubmed|15699190,1903432,8755877]

Biological materials

Mutant

• BKE23390 (Δ[gene|CBE5093DFBBCE25071CCE12408BC9AFF7A158C03|spoVAF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACGGTTTTTGAAATACTCTT, downstream forward: _UP4_TAATCGCATTGAAACTGACT
• BKK23390 (Δ[gene|CBE5093DFBBCE25071CCE12408BC9AFF7A158C03|spoVAF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACGGTTTTTGAAATACTCTT, downstream forward: _UP4_TAATCGCATTGAAACTGACT

References

• 3114420,11751839,15451103,3926949,17158659,6432957,7934830,22328679,15699190,1903432,8755877,21398556,24682327,26731423